Stat1 chip
WebMonoclonal Antibody for studying Stat1 (Tyr701) phosphate. Cited in 836 publications. Validated for WB, WB, IP, IHC, IF, F, ChIP, ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Stat1 (Tyr701) (58D6) Rabbit Monoclonal Antibody (CST #9167) is ready to ship. WebJul 25, 2024 · We next performed STAT3 ChIP-seq experiments and analyzed binding of STAT3 and STAT1 ( GSE43036) 8 to enhancers in clusters e1-e5 after stimulation of human macrophages with LPS, IFN-γ, or...
Stat1 chip
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WebThe Stat1 Antibody confirms silencing of Stat1 expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity. Show More. Western blot … WebApr 30, 2024 · Sphingosine 1-phosphate receptor 1 (S1PR1) plays a pivotal role in mediating trafficking and migration of immune cells. Previous reports also identify S1PR1 as an …
WebChIP. Chromatin immunoprecipitations were performed with cross-linked chromatin from HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either Phospho-Stat1 (Ser727) Antibody or Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using … WebSTAT3 belongs to the STAT family of transcription factors consisting of seven proteins, namely, STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b and STAT6. Activated STAT3 translocates into the cell nucleus and binds to the interferon-gamma activated sequence ... STAT1: ChIP: −604 to −596 bp −444 to −435 bp −363 to −356 bp −246 to − ...
WebMonoclonal Antibody for studying Stat1 (Tyr701) phosphate. Cited in 325 publications. Validated for WB, IP, IF, F, ChIP, ChIP. Available in 3 sizes. Highly specific and rigorously … WebChIP- qPCR analysis of STAT1 (pSer727) was performed with 10 µL of the STAT1 (pSer727) Rabbit polyclonal antibody (Product # 44-382G) on sheared chromatin from 2 million HeLa cells treated with 50 ng/mL of IFN Gamma for one hour using the MAGnify™ Chromatin Immunoprecipitation System (Product # 49-2024). Normal Rabbit IgG was used as a ...
WebPhosphorylation of STAT1 regulates STAT1 activity. Phosphorylation of Tyr701 by Janus kinases (JAKs) triggers STAT1 homo- and hetero-dimerization with other STAT family …
WebSTAT1 is required for signaling of all the interferons (α, β, γ, ω, λ). Complete (recessive) loss of activity of STAT1 leads to early fatal infections with mycobacteria and viruses. … subtitle provocation movieWebMar 22, 2024 · THIF inhibited STAT1 phosphorylation and might bind to the STAT1 SH2 domain, leading to blockade of STAT1-STAT1 dimerization. ... and the anti-STAT1 antibody was used for ChIP-qPCR to measure the binding of STAT1 to the promoters. Data were analyzed and are plotted as percent (%) of input DNA. *p < 0.05 versus vehicle; **p < 0.01 … subtitle pursuit of happinessWebMay 11, 2024 · Chromatin immunoprecipitation (ChIP) of STAT1-associated DNA in RAW 264.7 macrophages revealed this region, located ~800 base pairs downstream of exon 1 … painted cave preserveWebFeb 13, 2024 · ChIP-seq and ChIP-qRT-PCR analyses were used to detect the downstream of STAT1. Nestin-CreERT2::Ai9 transgenic mice were used to genetic lineage tracing of Nestin + NSCs after SCI in vivo. Results A prolonged increase of activated CD8 + T cells occurs in the injured spinal cords. subtitle pro after effectsWebNov 26, 2024 · The C-terminal transactivation domain of STAT1 is required for an efficient recruitment of the components of the core Mediator complex to the IRF1 promoter, which harbors an open chromatin state under basal conditions, inducing transcriptional activation . Using ChIP assays, we found that IRF1 binds to the Mda5 promoter. painted cave mountain lionWeb【派森诺多组学项目文章】转录组+ChIP-seq+ATAC-seq共同揭示BRD9在破 上海派森诺生物科技股份有限公司 04-12 09:18 投诉 阅读数：159 subtitle qc meaningWebMay 15, 2024 · Of the 34 loci known to contain STAT1 interferon-responsive binding sites, ChIP-seq found 24 (71%). ChIP-seq targets were enriched in sequences similar to known STAT1 binding motifs. Comparisons with two ChIP-PCR data sets suggested that ChIP-seq sensitivity was between 70% and 92% and specificity was at least 95%. Use ChIP-seq to … painted caves ultima online