Lightshifttm poly di-dc
WebAug 2, 2024 · One of the most potent, and likely the most widely used, nonspecific competitor is the synthetic polymer poly(dI-dC)•poly(dI-dC). Its addition to the reaction mixture prior to crude nuclear proteins has unquestionably proven very efficient in reducing nonspecific interactions by facilitating detection of the complexes of interest. WebAt high salt, Poly (dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. [ 4] It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA). [ 5] Safety Information Storage Class Code 11 - Combustible Solids WGK WGK 3 Flash Point (F) Not applicable Flash Point (C)
Lightshifttm poly di-dc
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Web"polydeoxyinosinic polydeoxycytidylic acid, sodium salt", poly(dI-dC).poly(dI-dC) Section 2 - HAZARDS IDENTIFICATION CHEMWATCH HAZARD RATINGS Min Max Flammability: 1 Toxicity: 1 Body Contact: 0 Reactivity: 0 Chronic: 0 Min/Nil=0 Low=1 Moderate=2 High=3 Extreme=4 CANADIAN WHMIS SYMBOLS None EMERGENCY OVERVIEW RISK … WebPoly(deoxyinosinic-deoxycytidylic) acid sodium salt has been used in the electrophoretic mobility shift assay in p65 protein and neuro-2A protein lysate. It has also been used as a …
WebPoly (dI•dC), which is included in the kit, is the nonspecific competitor DNA of choice for most systems. However if the Test System target DNA sequence is GC-rich, try Poly …
WebPoly(dI:dC) (12.8 A 260 units/mg) and poly(dG:dC) (8.0 A 260 units/mg) were dissolved separately in NET buffer (legend to Fig. 1) to of 2.1 g/L and warmed up to 45°C for 5 min to ensure the ... WebPoly (dimethylsiloxane-co-diphenylsiloxane), dihydroxy terminated Linear Formula: HOSi (C6H5)2O [Si (CH3)2O]x[Si (C6H5)2O]ySi (C6H5)2OH CAS No.: 68951-93-9 Match Criteria: Product Name, Keyword All Photos ( 1) Poly [9,9-bis- (2-ethylhexyl)-9H-fluorene-2,7-diyl] Synonym (s): Poly [9,9-di- (2′-ethylhexyl)fluorenyl-2,7-diyl] CAS No.: 188201-16-3
WebThermo Scientific™ LightShift Chemiluminescent EMSA Kit is an extraordinarily robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions.
WebLightShift poly(dI-dC) can be used as competitor for nonspecific DNA binding proteins.Nucleic acids or proteins in a complex sample can be visualized using … crafts using toilet paper tubesWebTo achieve stimulation of cytosolic DNA sensors, Poly (dG:dC) must be delivered into the cytoplasm, for example by using a transfection agent, such as LyoVec™ . Key features of Poly (dG:dC): Poly (dG:dC) is a cytosolic DNA sensor … crafts using toilet paper rolls for kidsWebThermo Scientific LightShift Poly (dI-dC) can be used as competitor for nonspecific DNA binding proteins. More Product Data • Transfer EMSA gels using the Pierce G2 Fast … Thermo Scientific LightShift Poly(dI-dC) can be used as competitor for nonspecific … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes dixie state clock towerWebThermo Scientific™ LightShift™ Poly (dI-dC) Identify and characterize protein-DNA binding interactions by EMSA with this extraordinarily robust and sensitive system. Supplier: … dixie state football scoreWebThermo Fisher lightshift poly di dc Lightshift Poly Di Dc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - … dixie state bomb threatWebOct 1, 1988 · Dot blot e.l.i.s.a. conditions are described which allow quantification of Z-DNA [Br-poly(dG-dC).poly(dG-dC)] within the range 5-250 ng. Dot blot and transblot horseradish peroxidase e.l.i.s.a. are described that detect Z-DNA within supercoiled plasmid DNAs immobilized on diazophenylthioether (DPT) paper. crafts using vintage hankiesWebJan 1, 2013 · Addition of poly dI-dC minimizes nonspecific binding . The binding mixture must be kept in ice at all times. 14. A gel pre-run is strongly recommended. The casted gel is mounted with clamps on a vertical electrophoresis apparatus. Wells are gently washed with the running buffer (1× TGE) and, then, loaded with 5 μL of 1× gel loading buffer. crafts using vintage jewelry