Fred2/optitype
WebAug 20, 2014 · OptiType (v1.3.3) [37] was performed on germline pairedend whole-genome sequencing fastq files after fishing for HLA reads at 95% identity, taking only the top match with razers3 (v3.5.8) [38 ... OptiType is a novel HLA genotyping algorithm based on integer linearprogramming, capable of producing accurate 4-digit HLA genotyping predictionsfrom NGS data by simultaneously selecting all major and minor HLA Class I alleles. See more OptiType uses the following software and libraries: 1. Python 2.7 2. RazerS 3.4 3. SAMtools 1.2 4. HDF5 1.8.15 5. CPLEX 12.5or other Pyomo … See more Optional step zero: you might want to filter your sequencing data forHLA reads. Should you have to re-run OptiType multiple times on the same sample(different … See more
Fred2/optitype
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WebOptiType/OptiTypePipeline.py. from NGS data by simultaneously selecting all minor and major HLA-I alleles. and their dependencies. in the configuration file for RazerS 3.1. CPLEX should be globally executable. … WebMay 10, 2024 · docker pull fred2 /optitype opitype的使用分成以下两步: 1. 采用 razers3 比对参考序列数据库 razers 是一款比对工具,可以从原始的测序数据中筛选出属于HLA基因的reads。 opitype利用HLA I型基因exon2到exon3的DNA序列构建了一个参考数据库,如果reads能够比对到这个参考数据库,认为这些reads来自与HLA基因。 用法如下
WebNGS-based HLA typing algorithm. Container. Pulls 10K+ Overview Tags. Dockerfile ################################################################# # …
WebSep 9, 2024 · If it's used in the pipeline, it comes in the pipeline container (s) (or conda environment or whatever packaging system you use). So you should never have to touch … WebFeb 17, 2024 · I tried bootstrapping the singularity image as follows: singularity build optitype.simg docker://fred2/optitype However, this does not seem to run as expected. …
WebParameters: ngsFile (str) – The path to the NGS file of interest; output (str) – The path to the output file or directory; command (str) – The path to a alternative binary (if binary is …
WebDec 1, 2014 · OptiType: precision HLA typing from next-generation sequencing data Bioinformatics. 2014 Dec 1;30 (23):3310-6. doi: 10.1093/bioinformatics/btu548. Epub 2014 Aug 20. Authors András Szolek 1 , Benjamin Schubert 2 , Christopher Mohr 2 , Marc Sturm 1 , Magdalena Feldhahn 1 , Oliver Kohlbacher 1 Affiliations dead by daylight horror game ps4WebFeb 26, 2016 · Here we present FRED 2, an open-source immunoinformatics framework offering easy and unified access to methods for epitope prediction and other … gemstone chips beadsWebDec 19, 2024 · I did the following and so far optitype is running: sudo adduser biodocker sudo chown -R :users /work/Projects sudo chmod -R 777 /work/Projects sudo docker run -v /work/Projects:/data -t fred2/optitype -i EA005_001_1.fq EA005_001_2.fq -d -o /data dead by daylight hoursWebJan 1, 2016 · FRED 2 (framework for epitope detection) is an opensource framework designed to address the issue of the large number of pipelines and tools that each require unique inputs and provide different... dead by daylight - hour of the witch chapterWebDec 1, 2014 · We present OptiType, a novel HLA genotyping algorithm based on integer linear programming, capable of producing accurate predictions from NGS data not … gemstone charms wholesaleWebParameters: ngsFile (str) – The path to the NGS file of interest; output (str) – The path to the output file or directory; command (str) – The path to a alternative binary (if binary is not globally executable); options (str) – A string with additional options that is directly past to the tool; delete (bool) – Boolean indicator whether generated files should be deleted afterwards gemstone chips wholesaleWebApr 14, 2024 · 接下来要修改Optitype文件夹里的config.ini文件。. 复制一个example来修改即可(其实除了razers的路径和线程数,没啥好改的,glpk只能单线程运行,修改线程数没用)。. Optitype会调用razers进行比对。. cp config.ini.example config.ini. 软件的运行. python OptiTypePipeline.py \ -i sample ... gemstone chamber how to craft