Dh5 alpha subcloning efficiency
WebSubcloning Efficiency™ DH5α™ Chemically Competent E. coli Catalog no. 18265-017 General Information and Kit Contents Shipping and Storage The kit is shipped on dry ice. … WebEconomical for routine subcloning procedures or applications where starting DNA is not limiting. Manufacturer: Invitrogen™ 18265017. Catalog No. 18-265-017. $101.00 / Each …
Dh5 alpha subcloning efficiency
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WebSubcloning Efficiency™ DH5α™ Competent Cells are tested for transformation efficiency using the pUC19 control DNA supplied with the kit and using the protocol on page 2. … WebDH5α™ is a well-known, versatile strain that can be used in many everyday cloning applications. In addition to supporting blue/white screening, rec A1 and ... • Subcloning Efficiency DH5α Competent Cells: 4 vials, 500 µl …
WebDec 13, 2011 · E. coli. (Subcloning Efficiency)? The properties of this strain that contribute to its usefulness as a cloning strain are described below. The genotypes underlying … Web5 Using DH5α-T1R as a Transient Host Introduction DH5α-T1R competent E. coli support replication of M13mp vectors, but it does not support plaque formation. Plating on a lawn of E. coli containing the F plasmid will allow plaque formation. Before Starting You will need the following reagents: • Log-phase E. coli containing the F plasmid (e.g. DH5α-FT™, …
WebNEB 5-alpha Competent E. coli (High Efficiency) C2987: 6 x 0.2ml: Subcloning Efficiency ™ DH5α ™ Competent Cells* ThermoFisher (Invitrogen) 18265-017: 4 x 0.5ml: NEB 5-alpha Competent E. coli (Subcloning Efficiency) C2988: 6 x 0.4ml: MAX Efficiency ® DH5αF'IQ ™ Competent Cells* ThermoFisher (Invitrogen) 18288-019: 5 x 0.2ml WebMar 16, 2024 · Economical for routine subcloning procedures or applications where starting DNA is not limiting. Brand: Invitrogen™ 18265017. Additional Details : Weight : …
WebDH5 is a well-known, versatile strain that can be used in many everyday cloning applications. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5 increase insert stability and imp ...
WebDH5-Alpha Cells are E. coli cells engineered by American biologist Douglas Hanahan to maximize transformation efficiency. They are defined by three mutations: recA1, endA1 … rawlings wrist guardWebFor standard cloning experiments competent cells are available in two formats: high efficiency at greater than 10 8 cfu/μg for cloning (JM109, HB101), and subcloning efficiency at greater than 10 7 cfu/μg … simple guns to drawWebNEB 5-alpha Competent E. coli (High Efficiency) C2987: 6 x 0.2ml: Subcloning Efficiency ™ DH5α ™ Competent Cells* ThermoFisher (Invitrogen) 18265-017: 4 x 0.5ml: NEB 5 … rawlings wrs125WebE. coli. (High Efficiency) NEB 5-alpha Competent E. coli is a derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations. High efficiency strain ideal for a wide variety of applications. Available in a wide variety of sizes, including single-use vials, 200 μl vials, 96- and 384- well ... rawlings wpl95wnWebYour Price. C2988J. 6 x 0.4 ml. $106.00. $95.40. *On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca. NEB 5-alpha Competent E. coli is a derivative of … simple guy clown makeupWebDH5α Competent Cells for Subcloning are suitable for the standard DNA cloning applications: DNA cloning following PCR or restriction digestion reactions. Subcloning (transferring DNA from a parent vector to a destination vector) Plasmid isolation. Genotype: F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 … simple gym membership softwareWebDescription. Subcloning Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 10 6 cfu/μg … simple gut health diet