Webmay be cleaved while still bound to the gel with Thrombin or Factor Xa to liberate the protein of interest from the GST moiety. If the pGEX construct contains the recognition sequence for Thrombin refer to Appendix 5: Thrombin Cleavage of GST Fusion Proteins. 7 *Bed volume is equal to 0.5 X the volume of the 50% Glutathione Sepharose slurry used. Web使用强大的检测试剂盒来检测活化的小GTPase。
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WebCytiva Thrombin Protease. For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors. Brand: Cytiva 27-0846-01. Additional Details : Weight : 0.02340kg. Product Code. 10218854. 1486.87 SEK / 500 units. WebApr 10, 2024 · The ligated receptor was treated with thrombin protease to remove C-terminal His tag, and loaded onto a Superdex 200 column equilibrated in buffer (20 mM HEPES, pH 7.5, 100 mM NaCl, 0.01%MNG, 0. ... how is petroleum oil used
Thrombin VWR
WebCytovia Therapeutics Presents New Data on CD38-Targeted Flex-NK™ Bispecific Antibody at 2024 ASH Annual Meeting. December 9th, 2024. WebHRV 3C Protease is a highly purified recombinant protein derived from human Rhinovirus type 14 and expressed in E. coli. HRV 3C Protease cleaves a specific amino acid sequence (LeuGluValLeuPheGln ↓ GlyPro) and can be used to cleave fusion tag sequences from recombinant proteins that contain an HRV 3C protease cleavage site. More Overview WebSE 260 Minivertical Unit. SE 260 Mini-Vertical unit for two slab gels, complete. From 960.00 USD. SE 400 Vertical Unit. The sturdier vertical slab gel unit was designed with durability … how is petroleum gas obtained class 8